single-cell-clustering-and-batch-correction-with-omicverse（使用 omicverse 进行单细胞聚类和批量校正）

Single-cell clustering and batch correction with omicverse

Overview

This skill distills the single-cell tutorials t_cluster.ipynb and t_single_batch.ipynb. Use it when a user wants to preprocess an AnnData object, explore clustering alternatives (Leiden, Louvain, scICE, GMM, topic/cNMF models), and evaluate or harmonise batches with omicverse utilities.

Instructions

1. Import libraries and set plotting defaults

- Load omicverse as ov, scanpy as sc, and plotting helpers (scvelo as scv when using dentate gyrus demo data). - Apply ov.plot_set() or ov.utils.ov_plot_set() so figures adopt omicverse styling before embedding plots.

1. Load data and annotate batches

- For demo clustering, fetch scv.datasets.dentategyrus(); for integration, read provided .h5ad files via ov.read() and set adata.obs['batch'] identifiers for each cohort. - Confirm inputs are sparse numeric matrices; convert with adata.X = adata.X.astype(np.int64) when required for QC steps.

1. Run quality control

- Execute ov.pp.qc(adata, tresh={'mito_perc': 0.2, 'nUMIs': 500, 'detected_genes': 250}, batch_key='batch') to drop low-quality cells and inspect summary statistics per batch. - Save intermediate filtered objects (adata.write_h5ad(...)) so users can resume from clean checkpoints.

1. Preprocess and select features

- Call ov.pp.preprocess(adata, mode='shiftlog|pearson', n_HVGs=3000, batch_key=None) to normalise, log-transform, and flag highly variable genes; assign adata.raw = adata and subset to adata.var.highly_variable_features for downstream modelling. - Scale expression (ov.pp.scale(adata)) and compute PCA scores with ov.pp.pca(adata, layer='scaled', n_pcs=50). Encourage reviewing variance explained via ov.utils.plot_pca_variance_ratio(adata).

1. Construct neighbourhood graph and baseline clustering

- Build neighbour graph using sc.pp.neighbors(adata, n_neighbors=15, n_pcs=50, use_rep='scaled|original|X_pca') or ov.pp.neighbors(...). - Generate Leiden or Louvain labels through ov.utils.cluster(adata, method='leiden'|'louvain', resolution=1), ov.single.leiden(adata, resolution=1.0), or ov.pp.leiden(adata, resolution=1); remind users that resolution tunes granularity. - IMPORTANT - Dependency checks: Always verify prerequisites before clustering or plotting: # Before clustering: check neighbors graph exists if 'neighbors' not in adata.uns: if 'X_pca' in adata.obsm: ov.pp.neighbors(adata, n_neighbors=15, use_rep='X_pca') else: raise ValueError("PCA must be computed before neighbors graph") # Before plotting by cluster: check clustering was performed if 'leiden' not in adata.obs: ov.single.leiden(adata, resolution=1.0) - Visualise embeddings with ov.pl.embedding(adata, basis='X_umap', color=['clusters','leiden'], frameon='small', wspace=0.5) and confirm cluster separation. Always check that columns in color= parameter exist in adata.obs before plotting.

1. Explore advanced clustering strategies

- scICE consensus: instantiate model = ov.utils.cluster(adata, method='scICE', use_rep='scaled|original|X_pca', resolution_range=(4,20), n_boot=50, n_steps=11) and inspect stability via model.plot_ic(figsize=(6,4)) before selecting model.best_k groups. - Gaussian mixtures: run ov.utils.cluster(..., method='GMM', n_components=21, covariance_type='full', tol=1e-9, max_iter=1000) for model-based assignments. - Topic modelling: fit LDA_obj = ov.utils.LDA_topic(...), review LDA_obj.plot_topic_contributions(6), derive cluster calls with LDA_obj.predicted(k) and optionally refine using LDA_obj.get_results_rfc(...). - cNMF programs: initialise cnmf_obj = ov.single.cNMF(... components=np.arange(5,11), n_iter=20, num_highvar_genes=2000, output_dir=...), factorise (factorize, combine), select K via k_selection_plot, and propagate usage scores back with cnmf_obj.get_results(...) and cnmf_obj.get_results_rfc(...).

1. Evaluate clustering quality

- Compare predicted labels against known references with adjusted_rand_score(adata.obs['clusters'], adata.obs['leiden']) and report metrics for each method (Leiden, Louvain, GMM, LDA variants, cNMF models) to justify chosen parameters.

1. Embed with multiple layouts

- Use ov.utils.mde(...) to create MDE projections from different latent spaces (adata.obsm["scaled|original|X_pca"], harmonised embeddings, topic compositions) and plot via ov.pl.embedding(..., color=['batch','cell_type']) or ov.pl.embedding for consistent review of cluster/batch mixing.

1. Perform batch correction and integration

- Apply ov.single.batch_correction(adata, batch_key='batch', methods='harmony'|'combat'|'scanorama'|'scVI'|'CellANOVA', n_pcs=50,...) sequentially to generate harmonised embeddings stored in adata.obsm (X_harmony, X_combat, X_scanorama, X_scVI, X_cellanova). For scVI, mention latent size (n_latent=30) and gene_likelihood="nb"; for CellANOVA define control pools via control_dict. - After each correction, project to 2D with ov.utils.mde and visualise batch vs cell_type to check mixing and conservation.

1. Benchmark integration performance

- Persist final object (adata.write_h5ad('neurips2021_batch_all.h5ad', compression='gzip')) and reload when necessary. - Use scib_metrics.benchmark.Benchmarker with embeddings list (["X_pca", "X_combat", "X_harmony", "X_cellanova", "X_scanorama", "X_mira_topic", "X_mira_feature", "X_scVI"]) to compute batch-vs-biology trade-offs via bm.benchmark() and summarise with bm.plot_results_table(min_max_scale=False).

1. General troubleshooting

- Ensure adata.raw captures the unscaled log-normalised matrix before subsetting to HVGs. - Confirm use_rep='scaled|original|X_pca' strings exist in adata.obsm prior to clustering; rerun preprocessing if missing. - Monitor memory when running cNMF or scVI; adjust n_iter, components, or latent dimensions for smaller datasets. - Pipeline dependency errors: When you encounter errors like "Could not find 'leiden' in adata.obs", always check and add prerequisites: - Before leiden/louvain clustering → ensure 'neighbors' in adata.uns - Before plotting by clustering → ensure the cluster column exists in adata.obs - Before UMAP/embedding → ensure PCA or another dimensionality reduction is complete - Code generation pattern: When generating multi-step code, use defensive checks rather than assuming prior steps completed successfully. This prevents cascading failures when users run steps out of order or in separate sessions.

Examples

• "Normalise dentate gyrus cells, compare Leiden, scICE, and GMM clusters, and report ARI scores versus provided clusters."
• "Batch-correct three NeurIPS datasets with Harmony and scVI, produce MDE embeddings coloured by batch and cell_type, and benchmark the embeddings."
• "Fit topic and cNMF models on a preprocessed AnnData object, retrieve classifier-refined cluster calls, and visualise the resulting programs on UMAP."

References

• Clustering walkthrough: t_cluster.ipynb
• Batch integration walkthrough: t_single_batch.ipynb
• Quick copy/paste commands: reference.md